Generator
LacI:Cpn60

Part:BBa_K538201

Designed by: Bas Stringer   Group: iGEM11_Amsterdam   (2011-09-08)

pLacI + RBS + Cpn60

LacI:Cpn60
K538201


This part was submitted by team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011] as part of their project: icE. coli. Its purpose is to express the Cpn60 protein from Oleispira antarctica (BBa_K538001) under control of the Lac operon's promoter, pLacI (BBa_R0010). When coexpressed with Cpn10 (BBa_K538000), it should strongly increase E. coli 's ability to grow at low temperature. Coexpression can be achieved by transforming an E. coli strain comprising this BioBrick with a Cpn10 generator, such as K538200 and K538300, or by transforming cells with an operon-like BioBrick containing both the Cpn10 and Cpn60 coding regions, such as K538210, K538211, K538310 and K538311.


Refer to the main pages of the coding region parts (Cpn10: BBa_K538000 and Cpn60: BBa_K538001) for more information on how they facilitate cold resistance in E. coli.



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1096
    Illegal BamHI site found at 1162
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
//classic/generator/prc
//regulation/negative
Parameters
functionProtein expression
negative_regulatorsLacI
outputCpn60